It is worth considering anti-wrinkle treatment when lines and wrinkles begin to appear readily during regular or mild facial expressions, such as smiling or frowning. Usually, this begins to happen towards the late 20s and mid-30s but varies from person to person. It is best to start Botox treatment earlier than later to maintain a smooth, youthful appearance and delay the signs of aging by preventing further, deeper lines and wrinkles from appearing.